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Analysis of serpin and serpin-derived peptide inhibition of uPA, tPA, and PAI-1. Chromogenic assays for inhibition of serine proteases by 500 ng of Serp-1 or the RCL peptides are shown. A, inhibition of uPA activity; B, inhibition of tPA activity; C, inhibition of PAI-1 activity. D, molecular model showing S-7 docked in the β-sheet A of PAI-1. Error bars, S.E.
