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A, sequence alignments of SLC22 family members. Trans-membrane segments are highlighted in blue based on the TMHMM server predictions; N-glycosylation sites are colored in red. The sequence of the hLCN2-R-NTD construct used in this study is in italics and colored in light gray. B, canonic topology of the SLC22 family of organic cation transporters. C, proposed topology of LCN2-R. D, alternative topology proposed by Devireddy et al. (15).
