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Size exclusion chromatogram (280 nm absorption) of wild-type hLCN2-R-NTD (A) and hLCN2-R-QM (B). Samples where loaded on top of a Superdex 75 16/60 column and eluted by a flow of 1 ml·min−1 of 20 mm Tris, pH 7.4, 20 mm NaCl. C, calibration chromatogram, by order of elution: 67 kDa at 53 ml, 43 kDa at 59 ml, 13.7 kDa at 78 ml.
