Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of JNK inactivation on Akt phosphorylation in ceramide-treated C2C12 myotubes. A, cells were treated with 10 μmol/liter JNK inhibitor (SP600125) for 1 h prior to being incubated with 50 μmol/liter C2-ceramide (C2-cer) for 16 h. B, incubation of cells with 50 μmol/liter C2-ceramide for 16 h with or without SP6000125, followed by 100 nm insulin for 10 min. Cell lysates were immunoblotted with the indicated antibodies. The experiment was repeated twice in duplicates.
