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Effect of PKR inhibition on acute ceramide-induced inhibition of the insulin signaling pathway in C2C12 myotubes. C2C12 myotubes were treated with 50 μmol/liter C2-ceramide (C2-cer) for 2 h with or without imoxin (1 μmol/liter), followed by an insulin pulse before being lysed and immunoblotted with the indicated antibodies. The histogram represents densitometric quantification of Ser473 Akt.
