FIGURE 7.

Effect of PKR inhibition on chronic palmitate-induced inhibition of the insulin signaling pathway in human myotubes. A, PKR mRNA levels in skeletal muscle obtained from three diabetic patients compared with three healthy non-diabetic subjects. *, p < 0.05 relative to non-diabetic subjects. B, PKR phosphorylation state from skeletal muscle biopsy lysates obtained from two different diabetic patients compared with healthy non-diabetic subjects. C, cultured human myotubes were incubated with 0.5 mmol/liter palmitate (pal) for 96 h before being lysed. D, cultured human myotubes were incubated with 0.5 mmol/liter palmitate in the presence or absence of imoxin for 96 h, followed by 100 nmol/liter insulin for 10 min. Cell lysates were immunoblotted with the indicated antibodies.