FIGURE 4.

a, time course of glutathionylation of oxidized Prx2 by exchange with GSH. Oxidized Prx2 was incubated with GSH (8 mm), and conversion to glutathionylated species over time was followed by MS analysis as in Fig. 1. ●, disulfide (DS); ▴, dimer + one GSH (D+G); □, monomer + one GSH (M+G); ○, reduced monomer (M). Results are means and S.E. (error bars) from six to 10 independent experiments. b, Coomassie-stained non-reducing gel showing the time course of reduction of oxidized Prx2 with 8 mm GSH and where indicated 0.3 μm Grx1. c, Prx2 species distribution 20 min after treating oxidized Prx2 with 8 mm GSH and 250 μm H₂O₂. Samples were analyzed by LC/MS, and results are means and S.E. (error bars) from five independent experiments.