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Cys peptides detected after treating Prx2 with GSH and H2O2
Oxidized Prx2 (5 μm) was treated with 8 mm GSH and 240 μm H2O2 for 30 min, then blocked with NEM (15 mm). After 10 min, DTT (20 mm) was added to block residual NEM and reduce disulfides in the Prx2, and then iodoacetamide (50 mm) was added to derivatize the freed thiols. LC/MS/MS was performed on a chymotryptic digest. * represents position of IAM or NEM.
