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. 2016 Feb 5;7:54. doi: 10.3389/fpls.2016.00054

FIGURE 5.

FIGURE 5

Antigenicity of the N protein produced in plant by reaction with SARS patient sera. (A) Reactivity of the N protein produced in plant and in E. coli with SARS patient sera. Strips 1, 3: purified N protein produced in E. coli (500 ng); strips 2, 4: purified N protein produced in plant (250 ng). Strips 1 and 2: probed with a pool of 5 SARS patient sera. Strips 3 and 4: probed with the mouse anti-N pAb (positive control). (B) Validation of the results shown in panel A for the N protein produced in plant. Each strip derives from the blotting of a single-well SDS-PAGE gel (showed in C) loaded with 3.7 μg of purified N protein produced in plant. Each strip contains approximately 250 ng of N protein. Strip 1 was probed with the mouse anti-N pAb (positive control). Strips 2–7: probed with SARS patient sera (different pools deriving from several SARS patients). Strips 6–8: probed with pools of sera deriving from patients affected by non-SARS respiratory diseases (negative control). (C) Replica gel of the experiment shown in panel B stained by Coomassie Brilliant Blue for loading control of the purified N protein from plant.