Skip to main content
. 2016 Feb 4;7:10339. doi: 10.1038/ncomms10339

Figure 2. Y4 binds to hKCNK9 with subtype specificity and high affinity.

Figure 2

(a) Western blot analysis of hGH-hK2PM1P1 recombinant proteins blotted with Y4 and anti-hGH antibodies. Uncropped blots are shown in Supplementary Fig. 2b. (b) Flow cytometry analysis of HEK293 cells transiently expressing hKCNK9 or hKCNK3. (c) Sequence alignment corresponding to the M1P1 loop of hKCNK9 and mKCNK9 with non-conserved residues highlighted (top). Binding kinetics of Y-mAbs determined by Octet platform (bottom). (d) Y-mAbs' differential affinity to hKCNK9 versus mKCNK9 validated by western blotting of recombinant proteins and flow cytometry analysis of HEK293 cells transiently expressing hKCNK9 or mKCNK9. Uncropped blots are shown in Supplementary Fig. 2c.