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. 2016 Feb 3;7:10521. doi: 10.1038/ncomms10521

Figure 3. Loss of endothelial Dicer limits atherosclerosis.

Figure 3

(a,b) Atherosclerotic lesion formation in mice fed a HFD for 12 weeks analysed in aortic root sections stained with elastic van Gieson stain (a; n=8 mice per group) and in en face prepared aortas stained with Oil red O stain (b; n=9–10 mice per group). (c,d) Macrophage and smooth muscle cell accumulation in aortic root lesions determined by immunostaining of MAC2 (c, green; n=7–9 mice per group) and smooth muscle actin (d, red; n=8 mice per group), respectively. The nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI; blue). Scale bars, 500 μm (a), 50 μm (c,d) and 1 mm (b). Asterisks indicate the lumen. Dashed lines encircle atherosclerotic lesions. The data are represented as the mean±s.e.m. of the indicated number (n) of repeats. *P<0.05 and **P<0.01 by Student's t-test.