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. 2016 Feb 3;7:10637. doi: 10.1038/ncomms10637

Figure 5. Dendrimer-based nanoparticle delivery of siRNA into GSCs.

Figure 5

(a) Cellular uptake of Cy3-siRNA delivered by siRNA alone or G5 dendrimers (at N/P ratio of 5) to PBT003 cells. Scale bar, 200 μm. Assays were repeated three times. (b) Cellular uptake of Cy3-siRNA analysed by flow cytometry. Uptake of Cy3-siRNA delivered by G5 dendrimer complexes into PBT003 cells was 98.4%. (c) Expression of integrin αv and neuropilin-1 on PBT003 GSCs revealed by immunostaining. Scale bar, 100 μm. (d) Cellular uptake of Cy3-labelled siRNA delivered by siRNA alone, G5 dendrimers or RGDK-coated G5 dendrimers (at N/P ratio of 5) to PBT003 cells. Scale bar, 200 μm. Assays were repeated three times. (e) Knockdown of TLX expression in PBT003 cells by the G5 dendrimer-TLX siRNA complex (G5-siTLX) and RGDK-coated G5-siTLX, analysed by RT–PCR. G5 dendrimer-scrambled control RNA (G5-SC) and RGDK-coated G5-SC nanoparticles were included as controls. (f) Growth inhibitory effect of the G5-siTLX and RGDK-G5-siTLX. PBT003 cells treated with G5 dendrimers or RGDK-coated G5 dendrimers complexes with SC or siTLX (at the N/P ratio of 5) were analysed by CellTiter-Glo luminescent assay. N=3 for e; N=6 for f. For both e and f, *P<0.05, **P<0.01, ***P<0.001 by Student's t-test. Error bars are s.d. of the mean.