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. 2016 Feb 3;7:10637. doi: 10.1038/ncomms10637

Figure 7. TET3 regulates GSC self-renewal and tumorigenesis.

Figure 7

(a) Microarray analysis of PBT003 cells transduced with virus expressing scrambled control (SC) or TLX shRNA (shTLX). (b,c) RT–PCR analysis showing TET3 upregulation upon TLX knockdown. N=3, *P<0.05, **P<0.01 by Student's t-test. Error bars are s.d. of the mean. (d,e) Cell growth (d) and sphere formation (e) analyses of the GSC lines transduced with control RNA (shC) or TET3 shRNAs (shTET3-1, shTET3-2). (f) Schematics of TET3-1 and TET3-2 proteins with characteristic domains. (g,h) Cell growth (g) and sphere formation (h) analyses of GSCs transduced with control (C) or TET3-expressing lentivirus, TET3-1 or TET3-2. (i) RT–PCR analysis showing overexpression of TET3 in GSCs. (j) Relative sizes (volumes) of brain tumours derived from PBT003 cells that were transduced with control RNA (shC) or TET3 shRNA (shTET3). (k) Survival curves of NSG mice transplanted with PBT003 cells transduced with control RNA (shC) or TET3 shRNA (shTET3). X axis represents days after GSC transplantation. For d,g, N=4; for e,h, N=6. Error bars are s.e. of the mean. *P<0.05, **P<0.01, ***P<0.001 by Student's t-test. For j, N=3, ***P<0.001 by Student's t-test. Error bars are s.d. of the mean. For k, N=8, P<0.001 by log-rank test.