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. 2016 Feb 5;6:20273. doi: 10.1038/srep20273

Figure 2. HPLC profiles of biochemical assays of StnR, StnQ1, and StnK3.

Figure 2

(a) (i) l-Trp; (ii) β-methyl indolepyruvate; (iii) indolepyruvate; (iv) StnR incubated with l-Trp and α-KG; (v) StnQ1 incubated with indolepyruvate and SAM. (b) (i) l-Trp; (ii) (2S,3S)-β-MeTrp; (iii) (2S,3R)-β-MeTrp; (iv) StnR/Q1 incubated with l-Trp and SAM; (v) StnR/K3/Q1 incubated with l-Trp and SAM. (c) (i) (2S,3R)-β-MeTrp; (ii) (2S,3S)-β-MeTrp; (iii) StnR (20 μM) incubated with racemic β-methyl indolepyruvate and l-Trp; (iv-viii) StnR (2 μM) incubated with racemic β-methyl indolepyruvate and l-Trp for 0.5 h (iv), 1.0 h (v), 2.0 h (vi), 4.0 h (vii), 8.0 h (viii); (ix) l-Trp. ♦ S-adenosylhomocysteine (SAH) confirmed by MS.