Figure 3. Expression of PWS/AS locus genes in PWScr^{p−/m5′LoxP}, PWScr^p−/m+ and wild type mice.

(A–C) Northern blot analyses of PWS-locus snoRNAs from different tissues of wild type, PWScr^p−/m+ and PWScr^{p−/m5′LoxP} mice. Ethidium bromide-stained 5.8 S rRNA is shown as RNA loading control. Tissues and mouse genotypes are indicated on the top of each blot panel. (D) RT-qPCR analysis of PWS/AS-locus genes; the fold change is represented as 2^−ΔΔCq. Blue and red bars represent the RNAs expression fold change values of PWScr^p−/m+ and PWScr^{p−/m5′LoxP} mice, respectively. The plot represents values of Supplementary Table 2.