Figure 5. Snord116 in situ hybridization (ISH) of wild type and PWScr^{p−/m+5′LoxP} mouse sagittal brain sections.

(A) Brain sections of wild type mice, the exposure time was one day (B) brain sections of PWScr^{p−/m+5′LoxP} mice. ISH is performed with Snord116 antisense probe. (A,B) Mouse brain arias are denoted as follows: cc: corpus callosum; co: cortex; ha: hypothalamic area; hi: hippocampus; lv: lateral ventricle; on: olfactory nucleus; pu: putamen; ta: thalamic area; pir: piriformal cortex; il: infralimbic cortex; dp: dorsal peduncular cortex. Due to the lower expression levels of Snord116 in the KI mouse, the exposure time had to be increased from 1 to 7 days. (C) Schematic representation of the cuts in sagittal brain sections. (D) Negative control, example of brain sections of PWScr^p−/m+ mice hybridized with Snord116 antisense probe. (E) Example of brain sections of wild type mice hybridized with Snord116 sense probe. (D,E) The exposure time was 4 days.