Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 5;6:20113. doi: 10.1038/srep20113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Immunoblot analysis of SOX2, CDKN1A, CDKN1B and TP53 in the indicated lung SCC cell lines. The expression level of β-actin is shown as a control. (B) qPCR demonstrated that CDKN1A mRNA was significantly increased after SOX2 silencing by SOX2 siRNAs (siSOX2 #1 and siSOX2 #2) in EBC2, LK2 and H520 lung SCC cells. Non-targeting siRNA was used as a control (siCtrl). Detection of GAPDH was used for normalization. Results represent the mean ± SD (n = 3). Statistical significance was defined as p < 0.01 (*). (C) Immunoblot analysis shows increased CDKN1A expression 48 hours after SOX2 siRNAs (siSOX2 #1 and siSOX2 #2) transfection in EBC2, LK2 and H520 lung SCC cells. TP53 expression was not changed after SOX2 siRNAs transfection. Non-targeting siRNA was used as control (siCtrl). (D) The qPCR analysis showed that CDKN1A mRNA expression was decreased 48 hours after Ad-CMV/SOX2 (Ad-SOX2) infection at a MOI of 100 in EBC1 and at a MOI of 10 in H226 lung SCC cells. Ad-CMV/Luc (Ad-Luc) did not change CDKN1A mRNA expression in both lung SCC cell lines. Non-treated cells were used as a control (Ctrl). Immunoblot analysis demonstrated that CDKN1A protein was suppressed 48 hours after Ad-SOX2 infection in EBC1 and H226 lung SCC cells. Ad-Luc did not change CDKN1A protein expression in the cells. TP53 expression was not changed in the cells. Statistical significance was defined as p < 0.01 (*).