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. 2016 Feb 3;7:10580. doi: 10.1038/ncomms10580

Figure 2. Rescue of food anticipation in L Per2−/− mice.

Figure 2

(a) Western blot of liver extracts from mice held under restricted feeding conditions showing constant expression of PER2 in the liver of LPer2−/− mice after application of adenovirus expressing Per2. (b) Examples of double plotted wheel-running actograms under ad libitum (AL) and restricted feeding (RF) conditions. The black arrow and line delineate daily access to food. The blue arrow indicates adenovirus injection. avGFP, control adenovirus expressing green fluorescent protein (GFP). avPer2, adenovirus expressing Per2. (c) Quantification of wheel-running activity of n=6 animals for each genotype. Lower panel shows quantification of data from ZT2 to ZT4 (yellow area in upper panel). (d) Corticosterone levels in plasma (n=4). All values are mean±s.e.m. One-way ANOVA with Tukey's multiple comparison test, *P<0.05, **P<0.01, ***P<0.001. ANOVA, analysis of variance.