Table 1. Changes in amniotic fluid (AF), foetal, and placental weights (mg) at E13.5, E17.5 and E18.5.
| wild-type | Lrp4+/− | Lrp4−/− | |
|---|---|---|---|
| E13.5 | |||
| AF | 150.1 ± 7.6 (n = 5) | 162.4 ± 13.8 (n = 14) | 159.5 ± 4.0 (n = 5) |
| Foetus | 103.2 ± 10.6 (n = 5) | 108.8 ± 5.7 (n = 14) | 108.2 ± 4.5 (n = 5) |
| Placenta | 70.4 ± 8.5 (n = 5) | 67.8 ± 16.8 (n = 14) | 68.8 ± 11.9 (n = 5) |
| E17.5 | |||
| AF | 180.2 ± 52.0 (n = 9) | 218.6 ± 36.7 (n = 18) | 268.3 ± 44.1 (n = 11)*2, @ |
| Foetus | 835.5 ± 194.4 (n = 9) | 764.3 ± 159.1 (n = 18) | 775.8 ± 128.9 (n = 11) |
| Placenta | 111.8 ± 25.6 (n = 9) | 103.9 ± 19.7 (n = 18) | 107.7 ± 16.1 (n = 11) |
| E18.5 | |||
| AF | 124.0 ± 36.7 (n = 30) | 126.8 ± 37.0 (n = 49) | 351.2 ± 50.1 (n = 24)*3, @3 |
| Foetus | 1112.9 ± 104.1 (n = 30) | 1109.7 ± 103.9 (n = 49) | 1073.8 ± 62.0 (n = 24) |
| Placenta | 106.0 ± 14.0 (n = 30) | 108.5 ± 16.9 (n = 49) | 111.9 ± 16.2 (n = 24) |
| E18.5 Lrp4−/− | Morphologically Normal kidney | Unilateral kidney Agenesis | Bilateral kidney Agenesis |
| AF | 365.4 ± 48.6 (n = 7)*3,@3 | 339.7 ± 46.7 (n = 9)*3,@3 | 351.8 ± 58.0 (n = 8)*3,@3 |
Embryos at E13.5, E17.5, and E18.5 were obtained from a total of three, seven, and seventeen dam, respectively. Amniotic fluid weights of E18.5 Lrp4−/− embryos were divided into three groups by kidney agenesis. Values are reported as the mean ± SD (mg). *2P < 0.001 versus the wild-type, *3P < 0.0001 versus the wild-type, @P < 0.05 versus Lrp4+/−, @3P < 0.0001 versus Lrp4+/− by Tukey-Kramer’s post hoc test after a one-way analysis of variance. No significant differences were observed among the three groups of Lrp4−/− embryos.