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. 2016 Feb 4;7:10489. doi: 10.1038/ncomms10489

Figure 1. Rapid vesicular transport of TRPM8 via microtubules.

Figure 1

(a) TIRF images at consecutive intervals showing the movement of TRPM8–mCherry in TGN, control HEK293 cells and HEK293 cells treated with nocodazole (30 μM) and cytochalasin D (2 μM). The arrowheads point at moving TRPM8-positive structures. The rightmost column depicts the movement trace of the structures highlighted by the arrowheads. Scale bar, 10 μm. (b) TIRF images for control, nocodazole-treated and cytochalasin D-treated HEK293 cells illustrating the movement of TRPM8-positive structures in the indicated time interval, allowing calculation of the movement speed. The arrow indicates the direction and distance of movement. (c) Average speed of movement of the TRPM8-positive structures in control (n=26) cells and cells treated with nocodazole (n=20) and cytochalasin D (n=20) following the procedure depicted in b. (d) Quantification of the density of TRPM8-positive structures in control cells and cells treated with nocodazole and cytochalasin D. Significance was determined by 1-way ANOVA with Tukey's post hoc test; ***P<0.001.