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. 2016 Feb 4;7:10489. doi: 10.1038/ncomms10489

Figure 8. Reduced TRPM8 functionality in VAMP7−/− mice in vivo.

Figure 8

(a) Representative experiments depicting the positions of a VAMP7+/+ and a VAMP7−/− mouse on a thermal gradient plate during a 1-h experiment (left) and the corresponding presence time in the different temperature zones during the first and second 30-min periods (right). (b) Mean occupancy of the thermal gradient during 1 h by VAMP7+/+ (n =15) and VAMP7−/− (n=14) mice. (c) Mean distance covered on the thermal gradient by VAMP7+/+ and VAMP7−/− mice. (d) Comparison of the median temperature (Tmedian) in both genotypes during the first and second 30-min periods. (e) Comparison of the peak temperature (Tpeak) in both genotypes during the first and second 30-min periods. (f) Latency of the first nocifensive response on a 1 °C cold plate before (basal) and at 15 and 30 min after injection of left hind paw with 10 μl of a 5 mM icilin solution. Cut-off time was set at 120 s (dashed line). (g) Number of flinches on the 1 °C cold plate before (basal) and at 15 and 30 min after injection of the left hind paw. Significance was tested using two-way repeated measures ANOVA with Tukey's post hoc test. *P<0.05, **P<0.01.