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. 2016 Feb 5;6:20654. doi: 10.1038/srep20654

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Copyright © 2016, Macmillan Publishers Limited

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V5-tagged MsDorsal-RHD, Flag-tagged MsRel2-RHD, V5-tagged MsRel2A and MsRel2B (full-length proteins) were transiently expressed in S2 or Sf9 cells, and cytoplasmic (Cp) and nuclear (Nu) proteins were prepared separately for Western blot analysis using monoclonal anti-V5 or anti-Flag antibody. Panels A and B: DI-RHD-V5 and Rel2-RHD-Flag, respectively; Panel C: MsRel2A-V5 and MsRel2B-V5 from S2 cells.