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. 2016 Feb 5;6:20654. doi: 10.1038/srep20654

Figure 5. Moricin promoter is activated mainly by MsRel2-RHD.

Figure 5

The relative luciferase activities of different truncated moricin promoters (A) or different NF-κB-deleted or mutated moricin promoters (B) activated by recombinant MsDorsal-RHD (Dl-RHD) or MsRel2-RHD (Rel2-RHD) in Sf9 cells, or different constructs of moricin promoters (see panel E) activated by recombinant MsDorsal-RHD, MsRel2-RHD, or the two RHDs together (Dl + Rel2) in Sf9 cells (D) were determined by Dual-Luciferase® Reporter Assay System as described in the Materials and Methods. (C) The nucleotide sequences of the NF-κB sites from M. sexta moricin (Mor) and lysozyme (Lyz) promoters. The arrows indicate the direction of NF-κB sites. (E) Schematic diagrams of the moricin promoters (see text for detail information). Bars represent the mean of three independent measurements ±SEM.