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. 2016 Feb 5;6:20708. doi: 10.1038/srep20708

Figure 2. Feeder-free cultivation of hPSC on DAS-NG.

Figure 2

(a,b) Morphology of hiPSC seeded on (a) DAS/GL and (b) CVD/GL at day 1. (c,d) High magnification of hiPSC grown on (c) DAS/GL and (d) CVD/GL at day 3. (e–g) SEM images of hiPSC cultured on (e) MEF, (f) DAS/GL at day 3 and (g) Zoomed inset shows hiPSC (arrow) attached on graphene ridges (arrow heads) of DAS-NG. (h–j) Morphology of hiPSC colonies cultured on (h) DAS/GL, (i) MEF and (j) bare GL at 2 weeks. (k) The growth rates and mean doubling times (mDT) of hiPSC cultured on MEF, Matrigel or DAS/GL over 15 days. The points refer to the cell number of hiPSC every 3 days. The inset table represents mean doubling time. Data are presented as mean ± s.e.m (n = 3). (l) Percentage of OCT4+ hiPSC cultured on MEF or DAS/GL at P1, P5 and P9. Data are presented as mean ± s.e.m (n = 3). Scale bar, 200 μm (a–f), 1 μm (g), 1 mm (h–j).