Figure 3.

ET-1 is a miR-98 target gene. (A) Schematic illustration of the human ET-1 3′UTR, which contains a putative binding site (arrowhead) for miR-98. The miR-98 seed sequence is shown in bold font. (B) PAECs were transfected with pLightSwitch empty-vector (pLS-ET-1)-3′UTR (ET-1–3′UTR) luciferase reporter constructs or empty luciferase vector (VEC) + miR-98 mimic or scrambled miRNA (SCR) as described in Materials and Methods. After 72 hours, PAEC lysates were harvested and analyzed for luciferase activities. Each bar represents luciferase activity in relative light units as fold control versus PAECs transfected with VEC and treated with SCR construct (n = 3). *P < 0.05 versus SCR.