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. 2016 Jan;54(1):51–59. doi: 10.1165/rcmb.2014-0445OC

Figure 1.

Figure 1.

Nintedanib decreases constitutive expression of extracellular matrix proteins fibronectin and collagen 1a1 in idiopathic pulmonary fibrosis (IPF) fibroblasts. (A and B) IPF fibroblasts were treated with increasing doses of nintedanib (0.5, 1, or 2 μM) (A) or nintedanib (2 μM) for increasing durations (24, 48, or 72 h) (B). Expression of fibronectin and collagen 1a1 was evaluated by Western immunoblotting. (C) Densitometric analysis of independent experiments on different IPF fibroblast cell lines treated with nintedanib (2 μM) for 72 hours. Error bars represent mean ± SEM (n = 3). **P < 0.01. (D) IPF fibroblasts were treated with nintedanib (2 μM) for 72 hours, cells were lysed, and RNA was extracted. RT-PCR was performed to assess steady-state mRNA levels of fibronectin and collagen 1a1, compared with β-actin (control). Results are depicted graphically. Error bars represent mean ± SEM (n = 3). **P < 0.01. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.