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. 2016 Jan;54(1):51–59. doi: 10.1165/rcmb.2014-0445OC

Figure 5.

Figure 5.

Autophagy inhibition does not abrogate the effects of nintedanib on ECM proteins. (A) IPF fibroblasts were subjected to siRNA-mediated knockdown of beclin-1 and treated with nintedanib (2 μM) for 48 hours. Expression of ECM proteins (fibronectin and collagen 1a1) was evaluated by Western blotting. (B and C) Densitometric analysis of independent experiments performed as in A showing fibronectin (B) and collagen 1 (C) expression relative to GAPDH. Error bars represent mean ± SEM (n = 4). *P < 0.05; **P < 0.01. (D) IPF fibroblasts were subjected to siRNA-mediated knockdown of LC3 and treated with nintedanib (2 μM) for 48 hours. Expression of ECM proteins was evaluated by Western blotting. (E and F) Densitometric analysis of independent experiments performed as in D showing fibronectin (E) and collagen 1 (F) expression relative to GAPDH. Error bars represent mean ± SEM (n = 4). *P < 0.05; **P < 0.01.