Table 2.
Comparison of the Relative Expression of Selected Genes Determined by qPCR and Affymetrix U219 GeneChip Array Plate
| Gene Symbol | Relative Expression by Fold Change (n = 4) |
|||
|---|---|---|---|---|
| TGF-β1 Alone |
TGF-β1 Followed by PGE2* |
|||
| qPCR (±SE) | Array plate | qPCR (±SE) | Array Plate | |
| ACTG2 | ↑ 9.01 ± 4.01 | ↑ 6.23 | ↓ 36.82 ± 21.93 | ↓ 22.16 |
| RTKN2 | ↑ 4.84 ± 0.83 | ↑ 3.18 | ↓ 36.13 ± 13.28 | ↓ 15.24 |
| ORL1 | ↑ 1.91 ± 0.48 | ↑ 2.28 | ↓ 3.74 ± 1.24 | ↓ 6.96 |
| EDNRB | ↓ 7.17 ± 2.74 | ↓ 5.54 | ↑ 60.02 ± 22.02 | ↑ 45.25 |
| SOCS3 | ↓ 2.58 ± 0.38 | ↓ 4 | ↑ 8.10 ± 3.14 | ↑ 13.55 |
| NR4A2 | ↓ 7.44 ± 1.62 | ↓ 2.35 | ↑ 45.13 ± 25.40 | ↑ 24.76 |
Definition of abbreviations: PGE2, prostaglandin E2; qPCR, quantitative polymerase chain reaction; TGF-β1, transforming growth factor-β1.
*
Fold change by PGE2 relative to TGF-β1 treatment.