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. 2016 Jan;54(1):60–70. doi: 10.1165/rcmb.2015-0062OC

Figure 2.

Figure 2.

Suppression of poly I:C–induced CXCL10 by AF extract was mediated by protease-activated receptor-2 (PAR-2). Submerged NHBE cells were transfected with 10 nM of small interfering RNA (siRNA) against nonspecific control RNA or PAR-2. (A) Knockdown efficiency of PAR-2 mRNA was analyzed by RT-PCR. After transfection with siRNA against control RNA or PAR-2, cells were incubated with 1:320 wt/vol AF extract for 1 hour and then stimulated with poly I:C (5 μg/ml) for 6 hours. (B) Expression of CXCL10 mRNA was analyzed by RT-PCR. (C) Expression of CXCL10 protein in supernatant was analyzed by ELISA. NHBE cells were transfected with 10 nM siRNA against nonspecific control RNA or PAR-2. PAR-2 activator (AC-55541 100 nM, reconstituted in DMSO) was applied 1 hour before poly I:C stimulation. (D) Expression of CXCL10 mRNA was analyzed by RT-PCR. (E) Expression of CXCL10 protein in supernatant was analyzed by ELISA. Data represent mean ± SEM of three independent experiments. *P < 0.01 by t test when compared with medium-only control. #P < 0.01 by t test when compared with poly I:C–treated cells. &P < 0.01 by t test when compared with control siRNA-transfected cells.