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. 2015 Dec;53(6):834–843. doi: 10.1165/rcmb.2014-0376OC

Figure 2.

Figure 2.

KRIT1 knockdown attenuates PC-induced endothelial barrier enhancement, cortical actin remodeling, and p120-catenin–VE-cadherin interactions. (A) Pulmonary endothelial cells (ECs) were transfected with KRIT1-specific siRNA (siKRIT1) or with nonspecific RNA for 48 hours. EC permeability response to PC treatment (200 ng/ml) was monitored by measurements of transendothelial electrical resistance. (B) PC-induced actin cytoskeleton remodeling in control and KRIT1-depleted ECs was evaluated by fluorescence staining of F-actin using Texas Red phalloidin. Scale bar = 10 μm. (C) Effect of KRIT1 knockdown on PC-induced enhancement of adherens junctions was evaluated by staining for VEC. Results are representative of three independent experiments. nsRNA, control treatment with nontargeting RNA; si-KRIT1, treatment with KRIT1-specific siRNA.