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. 2015 Dec;53(6):834–843. doi: 10.1165/rcmb.2014-0376OC

Figure 6.

Figure 6.

KRIT1 deficiency increases HTV/TRAP6-induced lung vascular leak and attenuates protective effects of beraprost. Krit+/+ and Krit−/+ mice were pretreated with vehicle of beraprost (20 μg/kg, intravenously) followed by TRAP6 injection (1.5 × 10–5 mol/kg, intratracheally) and mechanical ventilation at high tidal volume (HTV) (30 ml/kg, 4 h) (TRAP/HTV). (A) Measurements of protein concentration in bronchoalveolar lavage (BAL) fluid. *P < 0.05 (n = 6). (B) Total cell count in BAL fluid. *P < 0.05 (n = 6). (C) Vascular leak was analyzed by Evans blue–labeled albumin extravasation into the lung tissue. Shown are images of lungs excised from the chest and perfused with PBS. (D) The quantitative analysis of Evans blue extravasation was performed by spectrophotometric evaluation of Evans blue extracted from the lung tissue samples. *P < 0.05 (n = 4). (E) Myeloperoxidase (MPO) activity in Krit+/+ and Krit−/+ mice exposed to TRAP6/HTV with and without beraprost treatment. *P < 0.05 (n = 4). ODU, optical density units.