Figure 1.

Immunofluorescence staining for IL-22R1 in rhesus monkey airway epithelium during the first year of life. Trachea cryosections from infant and adult monkeys were stained with a mouse monoclonal anti–IL-22R1 antibody (0.5 μg/ml) or a mouse IgG3 isotype control (0.5 μg/ml). Antibody binding was detected with Alexa 568–conjugated donkey anti-mouse antibodies (red). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Representative images from 1-, 6-, and 12-month-old animals are shown (n = 3–4 animals per age group). Isotype control as shown is for a 12-month-old animal. Scale bar, 25 μm.