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. 2015 Dec;53(6):853–862. doi: 10.1165/rcmb.2014-0462OC

Figure 1.

Figure 1.

Cystic fibrosis transmembrane conductance regulator (CFTR) activity contributes to microtubule formation rates. (A) Chronic inhibition of CFTR (20 μM CFTRinh172, 72 h) leads to decreased aster formations. Cells were immunostained for α-tubulin after microtubule formation assay. Representative images of seven experiments are shown. (B) S9 and IB3 cells were subjected to a microtubule formation assay and immunostained for α-tubulin. Representative images of seven experiments are shown. (C) Quantification of aster formation of chronic inhibition of CFTR (20 μM CFTRinh172, 72 h) was determined by the ratio of cells with asters/microtubule formations to total cells at various time points (n = 7; *P < 0.05). (D) For S9 and IB3 cells, aster formation was quantified, and the ratio of cells with asters to total cells at various time points (0–20 min) was graphed (n = 7; *P < 0.05). Data represent the mean (±SEM). NT = no treatment.