Figure 3.

Reduction of clock proteins along with sirtuin 1 (SIRT1) levels in peripheral blood mononuclear cells (PBMCs) from smokers and patients with COPD. (A) Levels of REV-ERBα and BMAL1 as well as SIRT1 in PBMCs from nonsmokers, smokers, and patients with COPD were measured by Western blot. Each group represents the results of five independent experiments, and the representative bands, which are demarcated by the lines based on groups, are from the same gel. (B) The relative band intensity normalized to GAPDH or β-actin is shown in the histogram. Data are shown as mean ± SEM (n = 3–4). *P < 0.05, **P < 0.01, and ***P < 0.001 versus nonsmokers.