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. 2015 Dec;53(6):844–852. doi: 10.1165/rcmb.2015-0015OC

Figure 2.

Figure 2.

IL-13 induces BDNF exon IV and VIb splice variants in human airway epithelial cells (HAECs) in air–liquid interface (ALI). (A) BDNF total (common exon IX) mRNA expression in HAECs in ALI (n = 11). (B) Semiquantitative gel PCR analysis of exons IV, Va, Vb, V-VIII-VIIIh, VIa, and VIb splice variants of BDNF stimulated by IL-13 in vitro (n = 3). (C and D) Quantitative real-time PCR analysis of BDNF exon IV (C) and exon VIb (D) splice variant mRNA induced by IL-13 in vitro (n = 11). (E) The ratio of BDNF exon VIb splice variant to total BDNF mRNA. *, exon Va; **, exon Vb; GUSB, β-glucuronidase; N.S., not significant.