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BDNF mRNA and protein expression in fresh bronchial epithelial cell brushings. (A and B) Quantitative real-time PCR analysis of (A) total BDNF mRNA and (B) exon VIb splice variant mRNA, respectively. (C) The ratio of BDNF exon VIb splice variant to total BDNF mRNA. (D) Total BDNF protein expression (by ELISA). Data are presented as median, 25th, and 75th percentiles with maximum/minimum whiskers.
