Figure 7.

The combination of rapamycin and resveratrol induces apoptosis in xenograft tumors. (A) Tumors were lysed as described in Materials and Methods, and caspase-3 and actin were detected by immunoblot. (B) Quantification of caspase-3 was performed using Image Studio 4.0 program and Excel. *P < 0.05; **P < 0.01. (C) Quantification of TUNEL-positive cells was performed as described in Materials and Methods. *P < 0.05. After immunohistochemistry, representative images for control (D), rapamycin (E), resveratrol (F), or the combination of rapamycin and resveratrol (G) were created using an EVOS Fl Auto microscope (original magnification: ×40). Arrows indicate TUNEL-positive cells. Scale bars, 100 μm. TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.