Table 1. Summary of the parameters defining centromere oscillation in bipolar and monopolar control, NuSAP, Kid, Nuf2, Nuf2/NuSAP and Nuf2/Kid siRNA cells.
| siRNA | Centre velocity (μm min−1) | Amplitude (μm) | Half-period (s) | ICD (μm) | No. of KT pairs/no. of cells |
|---|---|---|---|---|---|
| Bipolar | |||||
| Control1* | v1=1.68±0.06 | 1.12±0.08 | 32.50±3.34 | 0.75±0.09 | 298/12 |
| NuSAP | v2=1.39±0.03 | 0.78±0.11 | 32.56±3.55 | 0.69±0.09 | 226/8 |
| Kid | v3=1.54±0.06 | 0.79±0.05 | 31.73±4.14 | 0.71±0.10 | 344/11 |
| Nuf2 | v4=1.73±0.06 | 1.15±0.08 | 33.48±6.55 | 0.61±0.13 | 467/15 |
| NuSAP/Nuf2 | v5=1.46±0.03 | 0.92±0.06 | 33.24±2.93 | 0.60±0.08 | 257/11 |
| Kid/Nuf2 | v6=1.59±0.04 | 0.95±0.06 | 33.34±2.89 | 0.58±0.10 | 238/9 |
| Control2† | v13=1.70±0.11 | 1.18±0.12 | 33.83±4.36 | 0.73±0.08 | 263/9 |
| NuSAP/Kid | v14=1.32±0.08 | 0.63±0.15 | 32.69±5.46 | 0.63±0.07 | 408/10 |
| Monopolar | |||||
| Control1 | v7=1.39±0.10 | 0.69±0.04 | 31.06±2.88 | 0.57±0.11 | 358/15 |
| NuSAP | v8=1.25±0.06 | 0.66±0.04 | 30.65±2.87 | 0.56±0.07 | 261/10 |
| Kid | v9=0.76±0.02 | 0.49±0.03 | 28.66±2.22 | 0.52±0.08 | 447/13 |
| Nuf2 | v10=1.58±0.09 | 0.83±0.05 | 29.97±3.04 | 0.52±0.09 | 341/15 |
| NuSAP/Nuf2 | v11=1.03±0.03 | 0.56±0.05 | 26.96±2.17 | 0.47±0.08 | 237/11 |
| Kid/Nuf2 | v12=1.21±0.03 | 0.66±0.08 | 29.01±2.30 | 0.50±0.08 | 357/12 |
| Control2 | v15=1.40±0.11 | 0.62±0.09 | 32.60±4.81 | 0.56±0.07 | 410/11 |
| NuSAP/Kid | v16=0.74±0.04 | 0.47±0.10 | 30.35±4.05 | 0.49±0.07 | 384/12 |
ICD, intercentromere distance; Kid, kinesin-like DNA-binding protein; KT, kinetochore; NuSAP, nucleolar and spindle-associated protein; siRNA, short interfering RNA.
Parameter values are reported as the mean±s.e.m.
*Control1–scramble siRNA.
†Control2–double dose of scramble siRNA.