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. 2016 Feb 4;7:10543. doi: 10.1038/ncomms10543

Figure 6. Emiliania huxleyi and Gephyrocapsa oceanica are insensitive to Ge.

Figure 6

(a) Representative SEM micrographs for E. huxleyi and G. oceanica following treatment for 72 h in low Si (<0.1 μM) media with different additions of Ge (5 or 20 μM). No effect of Ge on coccolith morphology was observed in either species relative to the control grown in normal seawater media (100 μM Si). Si transporters (SIT/SITL) were not identified the genome of E. huxleyi or the transcriptome of G. oceanica. Scale bar, 2 μm. The results are representative of three independent experiments. (b) E. huxleyi cells were treated with 0, 0.5, 1 or 2 μM Ge for 48 h in seawater containing 2 μM Si. Mean specific growth rate and mean Fv/Fm as a measure of photosynthetic efficiency were determined. No significant differences were noted between treatments and the 0 μM Ge control (one-way ANOVA, n=3). The results are representative of two independent experiments. Error bars denote s.e.