. 2016 Jan 20;24(3):141–156. doi: 10.1089/ars.2015.6272

Table 1.

Disulfide-Linked Peptides of YchF Determined by Liquid Chromatography–Tandem Mass Spectrometry

Peptide A sequence	Peptide B sequence	Mass (Mr)/Da	Charge state	Identified in	Area^adimer	Area^amonomer
C⁵GIVGLPNVGK	AGIEAANFPFC³⁵TIEPNTGVVPM^*PDPR	3811.8703	3,4	Monomer	1.5E7	3.9E8
AGIEAANFPFC³⁵TIEPNTGVVPM^*PDPR	AGIEAANFPFC³⁵TIEPNTGVVPM^*PDPR	5514.6123	4	Dimer	1.5E8	5.9E6
AGIEAANFPFC³⁵TIEPNTGVVPMPDPR	AGIEAANFPFC³⁵TIEPNTGVVPM^*PDPR	5498.6171	4	Dimer	4.6E7	n. d.

Monomer and dimer bands were excised from the polyacrylamide gel and subjected to in-gel digestion using trypsin, followed by LC-MS/MS analysis. For the identification of disulfide-linked peptides, mass spectrometric data were analyzed using the xQuest/xProphet software suite. Quantitative data analysis was performed with the Skyline software.

^{^a}

chromatographic peak area summed for molecular ion MS1 isotope peaks.

M^*, oxidized methionine; n.d., not detected.