. 2016 Jan 10;24(2):99–112. doi: 10.1089/ars.2015.6347

Table 2.

Heme Oxygenase-1 Overexpression Prevents Br₂-Dependent Cytokine/Chemokine Induction

Cytokines	Role in lung injury	WT (pg/ml) n = 6	HO-1^−/− (pg/ml) n = 6	hHO-1 (pg/ml) n = 6
Bronchoalveolar lavage fluid
IL-1 alpha	Proinflammatory interleukin	14.69 ± 6.09	21.54 ± 3.95	19.51 ± 7.48
IL-6	Both pro- and anti-inflammatory	65.04 ± 21.25	1214.7 ± 517.44^a	69.64 ± 15.31^b
IL-9	Proproliferative and antiapoptotic	57.38 ± 9.51	55.92 ± 21.86	73.53 ± 20.61
IL-10	Anti-inflammatory	8.14 ± 4.48	12.45 ± 4.66	9.18 ± 5.45
IL-13	Induces airway diseases	12.48 ± 9.13	39.14 ± 21.56	24.07 ± 11.38
IP-10	Angiogenic chemokine	13.93 ± 1.71	69.10 ± 23.19^a	6.56 ± 0.43^b
G-CSF	Stimulates granulocyte release	1698.5 ± 722.04	5833.5 ± 2005.4^a	59.47 ± 8.32^b
KC	Chemoattractant for neutrophils	45.87 ± 13.27	428.63 ± 168.61^a	22.57 ± 4.58^b
MCP-1	Recruits monocytes	20.52 ± 5.34	162.65 ± 69.38^a	8.43 ± 1.51^b
MIP-1 alpha	Recruits leukocytes	18.08 ± 3.94	26.97 ± 6.71	14.05 ± 1.92
MIP-1 beta	Recruits leukocytes	9.59 ± 4.98	12.32 ± 3.00	1.40 ± 1.13
Plasma
IL-6	Both pro- and anti-inflammatory	1053.8 ± 519.41	4370.1 ± 1599.0^a	48.89 ± 21.10^b
IP-10	Angiogenic chemokine	89.59 ± 54.89	121.70 ± 45.32	25.56 ± 7.09
G-CSF	Stimulates granulocyte release	18369 ± 4598.8	16097 ± 4999.2	3199.2 ± 1454.9
KC	Chemoattractant for neutrophils	744.96 ± 221.15	1941.1 ± 674.08^a	217.55 ± 76.04^b
MCP-1	Recruits monocytes	67.00 ± 20.16	276.50 ± 104.39	21.73 ± 0.69

WT mice, mice with endogenous HO-1 deletion (HO-1^−/−), and mice overexpressing the human HO-1 gene (hHO-1) were exposed to Br₂ (600 ppm, 30 min), and then the cytokine/chemokine concentrations were measured in the BALF and the plasma 24 h postexposure. BALF and plasma cytokine/chemokine concentrations (pg/ml) are presented as mean ± SEM for groups (WT: n = 5–6, HO-1^−/−: n = 5–6, hHO-1: n = 5–6). All animals were males. Grayed rows indicate significant changes between HO-1^−/− mice and hHO-1 mice.

^{^a}

p < 0.05 versus WT.

^{^b}

p < 0.05 versus HO-1^−/−.

HO-1, heme oxygenase-1; MIP, macrophage inflammatory protein; WT, wild type.