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. 2016 Feb 1;30(3):257–265. doi: 10.1101/gad.274928.115

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© 2016 Patel et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — PRDM9_A DNA binding. (A) Domain organization of the hPRDM9 protein (accession no. Q9NQV7). (B) Crystal structure of hPRDM9_A (hPRDM9, allele A) ZnF8–12 in complex with the THE1B recombination hot spot sequence. ZnF8–11 (color-coded blue [ZnF8], cyan [ZnF9], orange [ZnF10], and magenta [ZnF11]) are shown in cartoon representation. ZnF12 was not visible in the structure. The helix of each finger forms specific H bonds in the major groove mainly with bases in the DNA strand (green) that is complementary to the consensus sequence (orange). The ZnF array, oriented N to C from left to right, interacts with this DNA strand oriented 3′ to 5′. (C) DNA sequences of THE1B hot spot (Myers et al. 2008), a predicted sequence (Baudat et al. 2010), and an arbitrary negative control that partially overlapped the consensus. The top line indicates the LD-hot spot motif (Myers et al. 2008), which was derived from recombination hot spots that are intrinsically sex- and population-averaged (Pratto et al. 2014). (D) PRDM9_A ZnF(8–12) binds the THE1B hot spot sequence.