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. 2016 Feb 1;30(3):281–292. doi: 10.1101/gad.274118.115

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© 2016 Coffill et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Partial conservation of the binding pocket between Mdm2 and p53 among vertebrate species. (A) Protein sequence alignment of the p53-binding region of Mdm2 from lampreys (this study), elephant sharks (Esharks, G9J1M1), zebrafish (Q561Z0), frogs (P56273), chickens (F1NGX6), mice (Q569X0), and humans (Q00987) (corresponding to human protein residues 1–110). (B) Protein sequence alignment of the Mdm2-interacting region of p53 sequences from lampreys (this report), elephant sharks (G9J1L8), zebrafish (P79734), frogs (P07193), chickens (P10360), mice (Q549C9), and humans (P04637) (corresponding to human protein residues 16–30). (C, top panel) Western blot showing in vitro translation and immunoprecipitation (IP) of Hs-p53 and Lj-p53 by either HDM2 or Lj-Mdm2, which was used as bait. Input levels can be seen in the bottom panel.