Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 1;30(3):281–292. doi: 10.1101/gad.274118.115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Coffill et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — Binding of Lj-p53 to HDM2 is enhanced by Lj-p53(G22L) mutation. (A) A surface presentation (pink) of the human crystal structure of HDM2^Nterm (residues 25–109) in complex with a fragment of Hs-p53 (cyan) showing the interaction of the three critical residues F19, W23, and L26. (B) A surface presentation (green) of a homology model of the p53-binding region of Lj-Mdm2^Nterm showing the putative interaction with Lj-p53 (orange). Note that the equivalent of L26 in Hs-p53 is a glycine, G22, in Lj-p53 (arrow in both figures). (C) Competition titrations of Hs-p53 and Lj-p53 peptides against FAM-12.1 for binding to HDM2^Nterm. K_D values are in Supplemental Table S2. (D) Western blot of Lj-p53 and Lj-p53(G22L) immunoprecipitated (IP) by HDM2^Nterm, which was used as bait. All proteins were expressed by in vitro translation. Input levels can be seen in the bottom panels.