Figure 5.

The DNA-binding region of Lj-p53 is conserved. (A) Protein sequence alignment of the DNA-binding domain of p53 from lampreys, elephant sharks (Eshark), zebrafish, frogs, chickens, mice, and humans. Accession numbers are in the legend for Figure 3B. Amino acids important for DNA binding and/or human cancer hot spot mutations (shown in red) are indicated by an asterisk. (B) Nucleotides representing the Hs-p53-binding consensus sequence ConA, which was used for DNA-binding and activation studies. Binding of in vitro translated Hs-p53Δ and Lj-p53Δ to the p21-2, PUMA-2, and ConA response elements. DNA binding corresponds to fold difference over nonspecific DNA binding between the respective p53 and equal molar concentrations of “no response element” control DNA. Error bars represent mean ± SEM of three independent binding experiments.