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. 2016 Feb 4;16:12. doi: 10.1186/s12896-016-0243-3

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© Kaushik et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Coomassie stained SDS-PAGE showing secreted EDIII. Presence of secretory EDIII was determined in the supernatant of cultures induced in the absence (CA 0 %) or presence of different concentrations of casamino acids (CA 0.25 % to 2.0 % w/v) supplemented BMM media from day 0 to day 6 at 20 °C (a) and 30 °C (b). For both panels, similar trends were observed in two independent induction experiments (Additional file 9)