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. 2016 Feb 4;16:12. doi: 10.1186/s12896-016-0243-3

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© Kaushik et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of temperature and CA supplementation on cell growth and culture pH. Cultures induced at 20 °C (a) and 30 °C (b) were observed for variation in growth profile (top) and pH (bottom) over time (days). Growth profile was determined by measuring the cell density for each time point at 600 nm. Cells were harvested and pH was measured for the retrieved cell-free media. Tables containing absolute OD₆₀₀ and pH values are also provided as additional information (Additional file 8: Table S8). Similar trends were observed in two independent induction experiments (Additional file 9)