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. 2016 Feb 4;16:12. doi: 10.1186/s12896-016-0243-3

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© Kaushik et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Evaluation of Immunogenicity of purified recombinant EDIII. a Coomassie stained 15 % SDS-PAGE showing purified recombinant EDIII as a ∼ 14 kDa band in five separate elution fractions (E1 to E5) after Ni-NTA chromatography. b Determination of anti-DENV serotype-3 neutralizing antibody titers using flow-cytometry based neutralization test. Mouse anti-EDIII pooled antisera (blue) and mock pooled antisera (red) were tested at various dilutions (X-axis) and percent viral infection (Y-axis) was calculated. The dotted line represents 50 % infection. The error bars represent standard deviation of two technical replicate