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Enzyme activities of recombinant Oc4CL1
| Substrate | V max (µM/s) | V max (pkat/ug) | K m (µM) | kcat (s−1) | kcat/K m (s−1 M−1) |
|---|---|---|---|---|---|
| Trans-cinnamic acid | 0.175 | 21.66 | 108.52 | 1.29 | 11,855.05 |
| p-coumaric acid | 0.356 | 44.06 | 16.42 | 2.62 | 159,386.65 |
| Caffeic acid | 0.546 | 67.57 | 79.88 | 4.01 | 50,249.26 |
| Ferulic acid | 0.917 | 113.49 | 87.2 | 6.74 | 77,308.62 |
The K m and V max of recombinant Oc4CL1 proteins were determined from a Lineweaver–Burk plot
