Table 2.
Summary of the scrapie transmission results in sheep transfused with PBMC, monocytes or T lymphocytes from preclinical sheep with classical ovine scrapie infection
| Group | Donor ID | Cell type | Cell numbera | Lamb PRNP | PrPSc detection by IHCb | sPMCA | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Antemortem | Postmortem | Antemortem | |||||||||||||||||
| Recipients | Controls | Animal ID | Rectal tissues | Rectal tissues | Other lymphoid tissuesc | Brains | PBMC | ||||||||||||
| 4 | 6 | 12 | 18 | 21 | 10 | 25 | 10 | 25 | 10 | 25 | 15 | 17# | |||||||
| 1 | 4454 | PBMC | 1.3 × 108 | VRQ/VRQ | 4599 | (−) | (−) | NA | NA | NA | (+) | NA | (+) | NA | (−) | NA | NA | NA | |
| VRQ/VRQ | 4601 | (−) | (+) | NA | NA | NA | (+) | NA | (+) | NA | (−) | NA | NA | NA | |||||
| 4438 | 4.5 × 108 | VRQ/VRQ | 4598 | (−) | (−) | NA | NA | NA | (−) | NA | (+) | NA | (−) | NA | NA | NA | |||
| VRQ/VRQ | 4600 | (−) | (−) | NA | NA | NA | (+) | NA | (+) | NA | (−) | NA | NA | NA | |||||
| NA | VRQ/VRQ | 4602 | (−) | (−) | NA | NA | NA | (−) | NA | (−) | (−) | (−) | NA | NA | NA | ||||
| VRQ/VRQ | 4603 | (−) | (−) | NA | NA | NA | (−) | NA | (−) | (−) | (−) | NA | NA | NA | |||||
| 2 | 4454 | Monocytes | 2.2 × 106 | VRQ/VRQ | 4594 | (−) | ND | (−) | (−) | (−) | NA | (−) | NA | (−) | NA | (−) | (−) | ND | |
| VRQ/VRQ | 4595 | (−) | ND | (+) | (+) | (+) | NA | (+) | NA | (+) | NA | (+) | (P) | ND | |||||
| 4438 | 4.5 × 106 | ARQ/VRQ | 4546 | (−) | ND | (−) | (−) | (−) | NA | (−) | NA | (−) | NA | (−) | (−) | ND | |||
| VRQ/VRQ | 4593 | (−) | ND | (−) | (−) | (−) | NA | (−) | NA | (−) | NA | (−) | (−) | ND | |||||
| VRQ/VRQ | 4597 | (−) | ND | (+) | (+) | (+) | NA | (+) | NA | (+) | NA | (+) | (P) | ND | |||||
| NA | VRQ/VRQ | 4596 | (−) | ND | (−) | (−) | ND | NA | (−) | NA | (−) | NA | (−) | (−) | ND | ||||
| 3 | 4454 | T lymphocytes | 5.9 × 106 | VRQ/VRQ | 4539 | (−) | ND | (−) | (−) | (−) | NA | (−) | NA | (−) | NA | (−) | ND | (−) | |
| VRQ/VRQ | 4590 | (−) | ND | (−) | (+) | (+) | NA | (+) | NA | (+) | NA | (+) | ND | (P) | |||||
| 4438 | 9.9 × 106 | VRQ/VRQ | 4588 | (−) | ND | (−) | (−) | (−) | NA | (−) | NA | (−) | NA | (−) | ND | (−) | |||
| VRQ/VRQ | 4589 | (−) | ND | (−) | (−) | (−) | NA | (−) | NA | (−) | NA | (−) | ND | (−) | |||||
| NA | VRQ/VRQ | 4591 | (−) | ND | (−) | (−) | ND | NA | (−) | NA | (−) | NA | (−) | ND | (−) | ||||
| VRQ/VRQ | 4592 | (−) | ND | (−) | (−) | ND | NA | (−) | NA | (−) | NA | (−) | ND | (−) | |||||
Abbreviations: PRNP prion genotype, PBMC peripheral blood mononuclear cells, NA not applicable, ND Not done, sPMCA Serial protein misfolding cyclic amplification, P positive for prion protein misfolding activity; #months post-inoculation; (+) = PrPSc immunolabeling was detected; (−) = PrPSc immunolabeling was not detected; aindicate the total number of PBMC, monocytes or T lymphocytes isolated from a 50 mL whole blood sample volume; bPrPSc immunolabeling in the tissues were detected using a mixture of prion mAbs F99/97.6.1 and F89/160.1.5 by immunohistochemistry; cOther lymphoid tissues include alimentary tract-associated lymphoid tissues (tonsils, retropharyngeal, mesenteric such as ruminal, abomasal, duodenal, jejunal, ileocecal, distal ileum, ileocecal junction lymph nodes, ileum, and spleens) and peripheral lymph nodes (prescapular, prefemoral, and popliteal)